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CRIMIC CR02345

Gene cbs

CG4840

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2R:13450586..13453393	50A9	FBti0213543	91515

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTGGAGGAGGAAATCGATGAACT	CTCCTCTCGTTCGATTTCCAGTTG

Images

Construct Design Sequences

sgRNA sequence	TATTAAACTGATTTAATCGTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
GTCCTCTTTGAGCGCAATAAATCTGTGAGTATGCCTCCCGATTCTGACGATTTCCATTGGGACTTGTAACACTAATTTAACCAACAATGCAGTACTAACTCTACCAATTAAGCTCCTGGTTTACTTGGAATTTTCCAACCACACCTATTATGTAGTTTTTGATTGTAAATGTTTTCCTTTTCGTATTAAACTGATTTAAT	CGTTGGCAAACTTTTTGGTAAACTTTTTCCTTCAATAATGCACGCATATATATGTTTACCCTCAATATCCAAAACTGATTGATGGAGATTTTGTAAACCAATAAACCGAATGTTCTCAGCAAAATTTGGAAACAGAGGTAAAAGATCTCAAGACTCGTCTCACGGCAGCGGATGACAGGTTCAGCGAGTACTCCTCGAAT

 

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