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CRIMIC CR02346

Gene Obp58b

CG13518

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2R:22704704..22705639	59A3	FBti0214886	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCGTTGCCATGACAAGATTCACTAGC	TAGTACGAGCGCACCTTGTCCATA

Images

Construct Design Sequences

sgRNA sequence	ATGGATTATCCACACTAGATCGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATTAGGGCTGTCCATCAAAGGGGAACCATTTGACATATAAAACTGAGGACGAACCGCTGGAAAGCTGTCATCATGCTGAGAATTGGTTTCGTGATTTGTGTGATTATATCACTGCGCTTGGTACGTACTCATAACACCGTTTTCAATTTGTTCTGGATTGGATAATGTTCGAGGGTGTTTTTAATGGATTATCCACACTA	GATCGGATTTATTGTACATAATAATTTTGAAAGTTCAAGGTCCATCGGGGATCCGTATTCGAGCTACTTTTTAATCCTTTAATATGTGCATATTTCTATGCAGAATGGACTCGTGGCTGTCCGAGTTCATTGCCGGCACATGGAGCGTATTCACGAGGAGAACATCCACCATTGCTGCAAACATCAGGATGGCCACGACG

 

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