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CRIMIC CR02352

Gene Tsp39D

CG8666

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2L:21344607..21358271	39D1	FBti0214889	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAAGCAGCCAGCGAGTAACGAAGCAA	GACGTGATCGCTAACGAAGTTCGA

Images

Construct Design Sequences

sgRNA sequence	TCGTCACTCTTTATCATACAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
GGCTTAACATGTGTAAAATACTTGACATTCTTTTGCAATCTTCTTTTTGCGGTAAGTCAATATCACTGACTATTTAATATAGTTGATTGCAATTTCAGCTTAAGTGGAGCTTTAATTTATATTTAAAAATCCTAATTTGTTTTTTTTTATAGTTAAGACACGGGGACCTCTGTGTACTTATCTTATTTTATATCCCTTGT	ATGATAAAGAGTGACGATTTTGAAATTTTTCTCATCTAAATCGGCAGTTTTTATCCTTTTTAATAATGTAATATTTTGGTGCTACATAGTGTTACCTTCGATTACAAAAACTTCAAAGCTTTAATTTAGATCACATTACTTGCCATTTATTCTTTTCATTAAGAGGACCTCTTAAAAAAGGTTTCTAAAATAAAAAAATT

 

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