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CRIMIC CR02353

Gene sNPF

CG13968

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2L:20027915..20056373	38B1	FBti0214890	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCATTGTGGAAAAGCGTTGGTTCG	GAAGAACCACTTTGGTTTGGGCAC

Images

Construct Design Sequences

sgRNA sequence	TGTGCTATATGCTACTCTAATGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCAGCAGCAGTTGGGTACCGAGCCGGACTCCGATCTGGGTGCCGACTACGACGGACTGTACGAGCGAGTGGTGCGGAAGCCACAACGACTTCGCTGGGGACGCAGTGTTCCTCAGTTCGAGGCAAACAACGCGGACAATGAACAAGTAATAATTTTCATTTCGTTTTGCAATACAAATAACCTTATTAATGAAACCATTA	GAGTAGCATATAGCACAATTTTAGCACCATTTTTAAAGTATAAAGATCTGGACATAGTTTTAATACTCTATACATTCCATTGGCCAGATTGAACGATCGCAATGGTACAACTCGCTTTTGAACTCGGACAAGATGCGGCGAATGCTAGTGGCCCTCCAGCAGCAGTACGAAATTCCGGAGAACGTGGCCAGCTATGCCAA

 

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