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CRIMIC CR02354

Gene CG5096

CG5096

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2L:10382263..10384723	31D11	FBti0214891	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGAGACGCCAAAGGTACCACATAA	GCGACAAATCGAGTGTGACTAGTTCG

Images

Construct Design Sequences

sgRNA sequence	GAAACCGACATATTAAGACATGG
sgRNA2 sequence

 

left_200bp	right_200bp
AAAAGTCTTGCAAAGCTAAATCGATAAAGATGACTCAAAGAAAGACCGAAAAATTCACCACACAAAAAATTTAAAAAAAAATGAGAACAGTGTGTAATATAAATAAGTTGTTTAAAAATCGCAATAAAATTTAATTTAATTTCTATGTTTGTCGAAGGTAAAATTAACCATCTAAAAATTAACGAAACCGACATATTAAG	ACATGGCAAAGCTATGTCCAAGAAACATTTAACTATATGGTAATCATTAGTTATTTAGTTCGCTATATAATACACACTACTTGCAGGAAAAAATCAAAGTAATTGATAGCAAATTGTGTAAGAAGTGCAATTGCTATATCGACACCAATTTGCTGGATTGCTCCGAAAAACTGCAGGATTGGTTGTCCGCCGAGGACTGG

 

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