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CRIMIC CR02358

Gene CG16974

CG16974

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2L:13183849..13191979	34A8	FBti0214894	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACTCGACGACCCACTAGACTATT	GGACTCGTACGAGAGTATCGATCA

Images

Construct Design Sequences

sgRNA sequence	AAATACATTTAGTCACCTCTAGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCCCAGTGCCGGATATCATCTGGGTGAGTATAACATCCTTGTATTGTACTCATATTTTGAGTTTATCGTGTTCGTTTAGGAGTAACTTAATGTTAAAAGAATATGTTCATATAGCATTGCATATTATTATATATTTAAATATCCCATGATATCGGAACTTATTATGCCCTTTTAATAGTTAATCCTTTAGCTTTCCTAGA	GGTGACTAAATGTATTTTTCTTCCATGAACTTATTATGAGGCACAGAGTTAATCGTTTACCCCTCATCAGGTGACACCGCGCAATAAGATTTTACGCCACCATGCTGATCCTGACAAGCGCCCGATCATCATCGACAGCAAGGAGGATGCTCACCAGCCACCGAGTGCCCAGGAGCTAGCTGCTCTGATGGACGAGAGCT

 

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