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CRIMIC CR02359

Gene Tsp42Ei

CG12843

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2R:7035125..7036949	42E5	FBti0213545	91517

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTGAGGCTCCTCATAGTAAGTAGC	TCCAGTAGTCCTCGGAACTGTTGA

Images

Construct Design Sequences

sgRNA sequence	TCTAAGCTGAATCATCGCATTGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCTAAAAGATATATATATTTTTTTTTTTAAACATATTTCCTGATTAAGTAATACTGTTTTAAGACAATATGCTATACAAACTCTTTATTTTATGTGGGTGCACATTATAGTACTGTCCTGCTTGGTCAGTGCTTTTTTGCACATTGCACCCATAAATTATAGAGTATGTTAGTAGCCTAAACGATTAGATTTAGCCAATG	CGATGATTCAGCTTAGATACTGATTGTAAGCTTAGTGAGAAGCGATTGTTTTCAGCAAAGCTGATTTGTAGATTTTGATTGCATCTATTAGATTTAAACTTGTCTCGGATATTTCCACTAATTTCTTCTAAATCCGATCCAAACCAATAGTATGTGGGAGCCGTGGTGCTGCTGATCCTCGCCCAGCTAATTTTTCTCGG

 

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