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CRIMIC CR02365

Gene stil

CG8592

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2R:12570367..12571883	49B5	FBti0214898	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGACGTCATAGTTCCTTGTTGGG	GGCAGTGTTTCCACCGAGATGTTT

Images

Construct Design Sequences

sgRNA sequence	GATTCTAACGGTAAAAGGCAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
CGGTTGCAGCCACAAGTACAGCTGCGCAGAGCGATTCCGGGGTGTTCGTGGACATGCAAGCTGCTGTTCCGTCTGGAAACGAAAGCCACGAGCTGAAAATCAAAACGGTTTTTCAGCGCCACAAGCAAGAGCAGGAGGGAGCTACCCGCAAGGTATGTTTTGGGTGGGCAGAAGCGAGATGTCGACATCTCTCACCTTGC	CTTTTACCGTTAGAATCGAATACTGTATCTGTTCGGATAGCGAAATAGCTTAATGTATTGTTGTGACATGTATTGGTGAGCTTGATATCTCCGTTTTTTTCCAGCTCGAAGATTCCACTTCAGATAAAGCTAATCTACCAAATATCCCTAAGAACTTGGGAATTGTCGTTCAGAACGTAAGTTTCCTTTATTCGCAAATA

 

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