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CRIMIC CR02367

Gene l(2)05714

CG8886

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2L:4970135..4973921	25B5	FBti0216176	92661

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCATCCAGGATTGAGTCGTTCGAG	ACGGATGAGGACAAGAAGGTGAAG

Images

Construct Design Sequences

sgRNA sequence	CATAGAGGGAGCTACTGGTTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
AGGAAAGTTTCAAGTACCACAACATTCTTGCCAAGGTGGGATTGGGTTTGTGCATAAGCAATAGGAATGACCCGTGTCCATTATTGATTCAAATATTTTGCGATCCAAACTAAATTTCAAACACGATTTTTGTGTGAGTTTTCGCCATACTTTTCGTTAACTATTCCATTGACTACTTCTTGTGCAATGGTTAACCAAAC	CAGTAGCTCCCTCTATGCGATGCACAACTTATCGCTCCAAAAAAAAAATTCTCATCTCATTACTAATTTACATAAAATTAACAACTCCGTATTTATACCTACAGAATATGATTAAGGGTGAACTTGTCAAAGATGAGCATGACCCAGACTTGGAGGCCTCCATAATTTCACCCAAAGCAAAGCCCAAAGCCAAATCAAAG

 

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