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CRIMIC CR02374

Gene Gr28a

CG13787

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2L:7452520..7454212	28A1	FBti0214901	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAATGCTAGTCTCGATCTGCTCGT	GGCATGCTGCTGTTCAACGTCATT

Images

Construct Design Sequences

sgRNA sequence	ACATTCAACTGGGACCTTGCTGG
sgRNA2 sequence

 

left_200bp	right_200bp
GTTTCTTTGCACCACGGACTTGGCCAGGAGCCGGTTTAGTATGTTAAACGAAGTGAGTGTCATGGGACTTTATTGCGCATGCAGTGGCATAATGTATGGAAAAAAAAAATGCGATGGATTAAATCACAAACTCTCTTCAAGTTAAATAGTTTGCAAACTTGGAGAGAATAATTTTTGTAGCATACTTTTTATAACCAGCA	AGGTCCCAGTTGAATGTGCATTATTTAGTGTAAAAGCTAGCTTATGATATATAATTTAATCTTTGACAGATCCTGCAGGATATTTTGGATGCCCATATAGAGCAATTAAGCGCCTTGGAACTCTCACCCATGCACTCGGTTGTCAATCACAGACGCTACTCACATCGCCTACGAAATTTGATTAGTACGCCAATGAAGCG

 

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