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CRIMIC CR02380

Gene Alp2

CG3290

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2R:22206005..22207987	58D1	FBti0214905	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCAGTGTGGTGGTGGTCACAAATC	GTGCACAATGTGTTGGAGTTGACG

Images

Construct Design Sequences

sgRNA sequence	CATGATAAAGAAAAAGGTTAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
AAAACGTGATAATGTTCCTCGGGGATGGTATGCCAATTAGCACAGTCACAGCTGCTCGCATCCTGAAAGGACAACGCCAAGGAAACACTGGGGAGGAGTCCTCACTGAGCTTCGAGAAATTCCCCTATTCGGGATTGAGCAGAGTGAGTGTTTGCATAGTACCATAGACCAGTAGTAATGATAGCAAAAACAAACCTTAA	CCTTTTTCTTTATCATGGTGAAGTTCACAACGTGTGCCAGGACCATAAAATCCCCACATAAGTGCTTTATTCAAACAAAAATATTATCTTTACAGACCTACTGCGCCAACGCCCAGGTTCCCGACTCAGCCTGTACTGCTACCGCTTATCTGTGCGGCGTTAAAACCAACATTATTAATATCGGAGTAAGTGCAGCAGTA

 

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