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CRIMIC CR02382

Gene Caper

CG11266

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2L:7032721..7037878	27D5	FBti0214906	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTTATCTGGGCAAACCATCTG	GTGCACGTTCAGTTTCATTGG

Images

Construct Design Sequences

sgRNA sequence	ACTCATGAGTTGTTGGTTTGGGG
sgRNA2 sequence

 

left_200bp	right_200bp
AGCAAACCACATTTTTCCGTAGCATTTACAAATGCAATTTTCGGTGTGTTGTGGAATAAATCTAATAAAATTATCATTAATTAACCCAAAACCAAAATCAGCATTATTGTCCGACAGCTCGCCAAGGTTTTAGTCAATGTAGTAGTCTATGACATCCAGCTAAAGTTGGAAATTAATTGAATTGAATTCTTTCGCCCCAA	ACCAACAACTCATGAGTTCAACGCTCCTATTTCTTCCTATCTGCTGTCTCACTAAGAACTACCGACAACTACCCACACACAATCACCATTAAAAAATACAAAAAAAAAAAAAAAAAAAAAAACCAAACAAAAATGCTAGAATAATTCTTAAGGGTGAAATGTCTTTGCAGAGAATCGAATAAGCTCAATATTGTTGCTAG

 

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