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CRIMIC CR02383

Gene Qsox1

CG4670

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2R:13231758..13235296	49F11	FBti0214907	92238

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GTGCCTACCTGGTATTGATTTCGC	GATGCCATTGCCTGTGAAACGCAA

Images

Construct Design Sequences

sgRNA sequence	TCTCTCTGAAACTTTAGGACTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTTCTGTTTTTCTGAACCTCTCGCTGAGCCCAGATTTTATTTGTTATTCACGTGAGTCACACAACAACTTTCTCTTAAGGCTAACTATACAAGTTATTTATTAGTAGGCTTCAAGGGCGAATTGTATTTATTGTATTTAAACAATCACTTACTTGTTTCATTTCAAATCAATGTTAGTTGTTTTCTCTCTGAAACTTTAG	GACTGGCTTGGCCTTTTAGAAAATGTTCTGTATTATTCTTGTAAATTAATGTGCAAATTTCTTGTAAATTTATGCCAAAAAAAATTTGAACTGTCTCGTTCGCACTAGCTGAGTAATGGGTATCTGATAGTCGATAGTGTTCTCTCTTGTTTCGGATTAAGGCTATAGTTAAGCCTCCCATAAATCTATTTTGTACCCAC

 

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