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CRIMIC CR02385

Gene BicC

CG4824

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2L:16042047..16048757	35E2	FBti0214908	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACCACGAGACTCCCTACATCAAGA	ATCTCCATCTGGACATTCACCAGG

Images

Construct Design Sequences

sgRNA sequence	TTATCAACTGATGTTACATTAGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTAATCATGGCATGTTACCAAATATTATTTGCATTTGACTATAATGGCTTGATTAGTTTAACTAATTTAGGTAAGATTTTGTAATATACATTCATACATACATTTAAGAGGGGGTATAGAAAATGTCAAATTTTTTTTTCCCAACCCTTGAGAGTTGTGATTATATATTGCTCCATTATAATTTTATCAACTGATGTTAC	ATTAGGCTAATTTTGCAGTTGTGTTATTATTATATATTTTTAAGTGATTTAGAACGCATTTAAAGTAATAAAGTTACTTAAACGTTATTTTCGAATTTGGCAACCTTAAATTACAACCAAGGATTTTTGTAAACAGTTTTTTCGTTGCTCTTAATTATTATTGAATATATTTTAAAGATTTTTCCTCCTTTCAGGTCATA

 

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