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CRIMIC CR02387

Gene qtc

CG14039

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2L:5060815..5070750	25C3-25C4	FBti0214909	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGCGTTGGCTTCTCAAACAGCTGA	TTTTGTTGCGTGGTCGCTGGTCAGT

Images

Construct Design Sequences

sgRNA sequence	TAAATACTTAATACCCCCCTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
GAGAAAATCAGTGAACACTAATAGCTTTGGTAGAATAGTTATTGAAATGATTTTGCTCCAGTTATAAATTGTAAATCGGAGAAATTCGTGCATCATTTGCAGAACTTAATGATTTAATCGTAGCACCACCGATTTAAGTCAGTCGGTTAGTCCGATTAGTCCGATTAGCAAAATGCTAAAAACTAAATACTTAATACCCC	CCTTGGAAAACGCAGACTAATGGTCAGTTAATTGACCACAAAAATGCTGCTCGATTCGTAGGAGCTGCAAGAAAAAAAAATATATAAAAAAAAGGGAGGGGATTTTCGAGAGCAGCACAACAATGAAAGTAAGCAAAGCCCGCCGCCAATGTTTTGTTAAATCTATGGCAACAAATTGCTTTTAGGGGCGTTGCAAATGC

 

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