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CRIMIC CR02390

Gene ms(2)34Fe

CG15287

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2L:14328429..14333860	35A4-35A2	FBti0214911	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTCGATGCGAGACGATGGTGTCAA	GATCGAGGTGTCCAGATGTAGATC

Images

Construct Design Sequences

sgRNA sequence	TACAGTTCCGGTGATTTCAGTGG
sgRNA2 sequence

 

left_200bp	right_200bp
CTGCGGTGCTATCACAACGATGGCACTGTCATTACTTCCGAAACCGCCGAAGGCTGGGATCCCGGCACGGATGTCGACGAAATTGACATGGAAATCAGTTCGAGCAGCTCACATGCGAATGTAGAAATCCAACCAAGCCACATATTCCGAAAGGTATGGTATATTCTCCAACATATTCTTGCCCAGCCTTTCTGCCACTG	AAATCACCGGAACTGTAGCTGCATTTCTTGGTATATTGACTTTTTGTTTTATACATTTTATTTGTCAGGAGACCATTTTTATCAACGATCCGGAAGGTTTGCCATTTAAAGATGTCAGCATAAGTAGTTCACAGGGTGTAGTTTCTCAAAGAATGAGTTTGAGAAGTAAAGAAATCTCTTCCAGTAGTGATGTCATGCCA

 

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