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CRIMIC CR02392

Gene ppk3

CG30181

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2R:23538631..23540773	59E3	FBti0214913	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCTTCACCGTCTGCATGGTGTACT	GGAAACGGTGTAAGTAGACCAGAC

Images

Construct Design Sequences

sgRNA sequence	GATGTTATCTAAGCACGTGCTGG
sgRNA2 sequence

 

left_200bp	right_200bp
GACGTCCAACCTGAAGGTACATATACATACATATAGACTCATCTTATCTGGGGAAAGTGGCCTGCGATTCGGTTCGGCGGGTAAACAAATTTCCCAACTCAAATGGAACAGCAGCTGATAAGTGGTATCAGTGCAAGGAACCCATATTTCACAGCAGCTTCCCTGGATTCGCTGGAGGAATGTGATGTTATCTAAGCACG	TGCTGGGATGCTCCACTTCATCACACCTATTAGAAACTTTCAGGTATCCATTTATTCCATTTATTCCACAGATCTACAGTAAGTCCCTGTGCCTGGCCAGATGTCGCGCTGTGATGGCCCTCGAGATGTGCAACTGTGTGCCCTTCTTCTACCCGTACGTGGATGGGCCCAGCTGCAATCCAGCCGGATTCGAGTGCCTG

 

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