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CRIMIC CR02395

Gene RnrL

CG5371

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2L:10325026..10327943	31D8	FBti0214915	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
multiple bands	OK	AATTGGACACTGCAATGCGAGCAG	GATCAACATGTCGCACGGTAACAC

Images

Construct Design Sequences

sgRNA sequence	GATGCGTATTTATATGTGATGGG
sgRNA2 sequence

 

left_200bp	right_200bp
CTATGACTGTTCTTAATATATTTATTTCAGATGGCAGGCAGGAGGAGGTGCACTTCGATAAAATAACCTCAAGAATCCAGAAGCTTTGCTACAACTTGAACATGGATTTTGTTGATCCTGTAAGTTTGTTTCTATTGTTTAAAGGTGCTCATCACACAGGTTGCACTCGAACCTAGTAAGTATGATGCGTATTTATATGT	GATGGGGTTTGATTTTATCTGCAACAGAATGTGCGAGCGCCCCTGGTGAAACTCATGTGGGAGTCCCAAGATATACAAACCATTTGTAATAAGTTATCAGCAGATTATTTGGGAAGTTTTCCTGATGTGTTATTATGCTTTATCTTTATAGGTTACTATCACCCTGCAAGTCATCAATGGTCTGTACTGCGGCGTGACCA

 

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