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CRIMIC CR02399

Gene Tsp42Eo

CG12838

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2R:7054717..7056576	42E7	FBti0214917	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGGCAGTCTGTCAAAGCACGATCA	TTGTAGTGGGACACCTTCCAGATG

Images

Construct Design Sequences

sgRNA sequence	GGCTCGTGGGATACGTCATCGGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTCCATTTCCACCAGACAAGATGTCACTTGGCGGCTCTCGTTCTGCCCACTTTTGTTGGCCAGTCGCATTTTCGGCACAATCGAAGCGACAGCAACAAGAAAGGAAACTGTGTATGTGCCATTTAAAAATAAAGCCCAAAGTAATTTAATTTATTTTTGCGCCCGGTTTGTTTTTATTTTGGTTGTTCTCGCTACCCGAT	GACGTATCCCACGAGCCGAAAGTTTGTCTCTGAAGATTTTGTTTGCCCATTTCATGGGCCATTAACAAACGCTTAATGTGCGCAGGCATAATTCATAATTTTAATACCGATCCAGATCGTCGGCGTCCTTGCGGCCCTGGCCGGCGTCTATGAGCTGGATAAGTTCAACGAGGGCTCTGCGGAGCACACAGAGAAGTTCG

 

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