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CRIMIC CR02400

Gene CG7222

CG7222

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2R:10813405..10815781	47C1	FBti0214918	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGCCTTTGGCACCGAATTCGCATA	GCATTCGGTGTCAGCCATTCTCTA

Images

Construct Design Sequences

sgRNA sequence	AACAGGCGGTATCTGACAATCGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTTACGTACGAGGAGGTGCGACGTATCGTCGAGGAGCTGGGTAATCAGTTCCGTGGCGACCGATATCATCTCATGAACAACAATTGCAACCACTTCTCGGGCTCATTAACTCAGGTGAGACATAAATCGAGGAAACTTTCCACTGGGTGGATCCAATATCAAGCAATATTTTGGGTGTGCTTATGCATGATTCACCGATT	GTCAGATACCGCCTGTTACAATCAGAAGTTCTTATTGTGAATCACCTCAAAAAGTAGGACAGATTACCATCCTAAACACGTCCAATACAATTAGATGCTTCCAAACGATTCGAGTTTAGGAGCAAGAAAATGCAAGCTTAAAAGCCCAAATGAATATTCATTTGACTATATCTGCAATTCCAATTACTCGTTCTAGATAC

 

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