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CRIMIC CR02401

Gene CG12164

CG12164

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2R:7311395..7313495	43A4	FBti0214919	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	TAGAAGACCTCAGAGGAGGCACAT	ACCTTCTTCCACTTGGTTTCCCAC

Images

Construct Design Sequences

sgRNA sequence	ACATAAACTTCAACCTTATGTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TCAGTGTCCGAGAAATACGAAAGATACCAGCTCCAAGATACCATAGTGCATCCTCGAGAAACAAACATGAAAACATTTGTGGAATTCGGAAATATATAAATGAAGTGAACATAACTGTGCAAAACCCAAAACTTAATAATAAAAAACACATAAAAATATCACATAAAACACCAAAGTCGCGTTGAAATTAAAGCCCACAT	AAGGTTGAAGTTTATGTCTTGGAACACAGCAAAGATTACAAAAATTACGAAATCGTACTTGAAAGTTAATTTAGACTTCTGATTCGAAACACAAATCTTTTGATCTAAGCATCTTTAATACGGAACAGAATCATCAGTTATCACTTTCAATCCAATAGTAAAATAGATATAAGCAAATACACATCATGGACTCAACAAAC

 

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