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CRIMIC CR02407

Gene Orcokinin

CG13565

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	2R:23946959..23949076	60A13	FBti0214922	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTTAGGAACTGTCTACTCTGCGTC	GCACAGTCATCTTCAAATCGCTCC

Images

Construct Design Sequences

sgRNA sequence	TTGTACAAAAGCTAAGCTGTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TCCAGTGGAAAGCTACGGGCTTCTGTAAACGGATTACAATTACTATAGCTAACTATGTTTTAGGATATTTCCAACGACGAACTGCTGGATGGAAAATATCTTTGTGAAGGTAACAACGAGCCATTGAACACATACTGAGAACCAACTTGTACATATATGTACATATATATACTCGAACTTTTTAATTATCATAACCAACA	GCTTAGCTTTTGTACAAAGCCATTAAGTCCTAATTTCTTATTATTTCGATTTCGATTTCGATCAGATCTGCCAGCGTTAAGAGAATTGTGTAACGGAAACAGCGTGAGTTTACGACAAACCGGACTTCCCAACGACGACCTTTCCACATCGCCGTTGTTTAACGAGTTTTACAAACTCTTCCAACGAAATGTAAATATAT

 

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