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CRIMIC CR02408

Gene CG30197

CG30197

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2R:14514030..14514992		FBti0214923	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	AGTTGTGCTCAACCGAATGTCCTG	CCAGTGCACAGAAAGTTGAAACACCC

Images

Construct Design Sequences

sgRNA sequence	AAATTTGGATATTAAAAAGAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
ACTACTACTACTACTACTACTACTATCTACTATAATAAGTATAATTGAATCGTGTTTAAAAGTGGCTTTTTGACTTTTCGAATTTAAAATCATCTTTGTGGAGAATCTTAATAATAAGGTACAATTTTTTGACCTTATTGAATTTTATGGAATCTTGATCTCCCTGTAATTTTTTTTTGGTTTTTTCTTTTTAACCTTCT	TTTTAATATCCAAATTTGAATAAAAAAAAAAATAATTTTTGTGTGCTTAATTTTCTAGCTGGAGCTACTGGCAGTTATTGCGGCAATGTCAAGTGCGGCAGCTTTGAGAAATGCGAAATGGATCGCAGCACCAAGCGACCCAAGTGCGTGAGATCGTGAAAACCAGGCAGCAAAATCAACGGACACGGCAGCAATATCAC

 

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