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CRIMIC CR02410

Gene sei

CG3182

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2R:24047411..24053035	60B5-60B6	FBti0214924	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGGAGATGTGCTCACCTCATTGT	TTGTCGGAGGACTTTGCTCTCAGGTA

Images

Construct Design Sequences

sgRNA sequence	CATAAGAAGTTATAGTTCGTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCCGGCAATATTGTTGTACTTGGTGAGTTCAGTGATTTCGGACCAACTGTCGTACATAAAATTAAACTCATAAATAAGACACTCTCTACCTCTTCTTTTTTTCTAGTTGTGAACTGTGTTCTATAGAGATATAGTTGCATGCTAGATTTTCCGTACATTCAGAGTTTACTTTAATTACTACTGCATAAGAAGTTATAGTT	CGTTGGCATTTGCAATTGATAATTAATCAAAACTCCTCTTGCTCAAGGTAAAAACGATATATTTGGCGAAAATCCGTGTATTTACCCAACGGTCGGAAAGTCGAACGGCGTGGTGAGAGCACTGACATACTGTGATATCCACAAACTGCACAGGGACGATTTGCTGGACGTGCTGGACTCGTATCCGGAGTTCCTGGAGA

 

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