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CRIMIC CR02411

Gene mthl15

CG31720

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2L:10234585..10238317	31B1	FBti0214925	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GATAAGCGAGCCATTGGACGGATT	GAACAGAAGTTTCTGGTGCGTGCT

Images

Construct Design Sequences

sgRNA sequence	CATTTGTCTGATCAAATAAGTGG
sgRNA2 sequence

 

left_200bp	right_200bp
CTAAATATCAACGCAAACTTCTCAAAGCCCTCAGGTATGTCGTCACCAGTTTTGCAGTTATTTTTATAGGTCTTTATAGCAGGAAACGAGCGGCAATTGAACGCAGTAATCGCCCATTAAAGTCGTAAACTTAATGATCAAAAATATTATTAAGAATTGGAAGCAATCGATTTTGATGTTTTCCATTTGTCTGATCAAAT	AAGTGGTATAAACAATTGGTTTCAAGTGCCAAGCAAATTTCGCTATTGAAGTTATCTACGTCATTCATTAGTATTGACATTTTTCATTAGCTTTGTCATTTTAATTATTTTTGTCAAATTTTCTTATCTGGTTAATCAACAAGTGAATGACACATTTGCCTTTTCATTGACATAGGTCAGATTGTATAGTGTATTGTATT

 

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