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CRIMIC CR02412

Gene CG30289

CG30289

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2R:21520413..21521848	57E9	FBti0214926	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
weak	OK	CAGCTTCGGACATACGAAGCAATG	CGCAACCATGGAAGTGATCAATGC

Images

Construct Design Sequences

sgRNA sequence	AAGTTAAGATATATATATCGTGG
sgRNA2 sequence

 

left_200bp	right_200bp
AACATTTTTGGAGGAGCCAAAACAAATATTCAAGAAAATCCTTGGATGGTGTTGGTTTGGTCGAGTAAGCCCTGCGGCGGCTCTCTTATTGCACGGCGTATGTATTTGACTAAAACATTGGTTATTCTTTATTTACTCTATACTAATATATTAATATATTTATATTTATTTAGATTTATAATATAGTTCATAGTCCACGA	TATATATATCTTAACTTACATGTACCCAAAAACTGAAAAGCATATGAATAAATAATCGAGATAGTCGACTATTTTATTATTTTTTTATTATTTATTTATTTTTCTTTTAAGAATTCGTCCTGACAGCAGCGCACTGCGTATCCTTTGAAGATCTGTATGTGCGCTTGGGTGATTACGAAACACTAGATCCCATGCCATAC

 

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