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CRIMIC CR02414

Gene AspRS-m

CG31739

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	2L:16827078..16832093	36B2	FBti0216975	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGTCACGTAAGTTCTCGTGAATCACG	GGATGCTTGTCTTCCAGAACCACA

Images

Construct Design Sequences

sgRNA sequence	GCAAGATATTTATAACCAGTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCGGTGGAGGCGGCGGTCGATTTGGTGGCCGTGGTGGCGGAGGCTATGCAGATGGTGGCGGCTACAATGACTTCGACAACTACCGTGGTGGCGGAGGACCAGGCTTCAATGACAACTTTGGACCAGGTCCCGGACCAAATCCTTTCAACAGTAAGTAGATAGCACTTAAAAGTAGCATCTTTAGTGGAATTGGTCCAACT	GGTTATAAATATCTTGCATAACGAATAGACAGTTTACAAAAACACTTATTTATTTTTTTTTACTTACATAGATATATTACATAAAATATTATTTTCACTCTTACATTTCCAGTGGGTGGTCCCAACATTTCTGGCAATGAGTTAATGCAGCTGATGAGTGCCATGGCCAGCAACTTCAATATGAATTCACAGCCCCCGCA

 

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