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CRIMIC CR02419

Gene Syx6

CG7736

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	2R:11219539..11222480	47D7-47E1	FBti0217652

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCCATACAGCAGCGTATGATCCAG	GACAGAACTCTGGAGCACTTGGTT

Images

Construct Design Sequences

sgRNA sequence	AAGTCACAAAAGTATTCAATGGG
sgRNA2 sequence

 

left_200bp	right_200bp
CAGGCCGTCATGCTAGACGACTTTGGGAACGAGTTCGATACCACGGAGTCCAAGTTGGATACGACCATGAAGAAAGTTGCCAAAGTCTTGCACATGAATAATGGTGAGTGGTGCGGCACTTGTGGTATTCAAGGACTATTTTGATTGAAATATTAATGAAGAGTAAATGCTTCAGTGAACCACAATCAAAATTACCCATT	GAATACTTTTGTGACTTCTGATTCCTAATTCTTAACTGTATCCATCTTCTTTCACAGATAAACGACAGTGGGCCGCCATTCTCATCCTATCCGGACTGTTATTGTTTGTGATAATTTTGTTTATTATTTTGTAATTGCTTTCGTTGTTCCCAAATCGTTTATATTTAGTGTAAATTAGATTTATTTATGACTTAGCTTGC

 

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