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CRIMIC CR02420

Gene l(2)03659

CG8799

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	2R:9390165..9395281	45D1	FBti0213551	91523

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGAGAACAATGGATTCGATCACCG	GGCACACAACCTGTTGCCAAGTAT

Images

Construct Design Sequences

sgRNA sequence	AGAAGATATAATATCAGTTTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
AATCTCTATCAGTTAACACTTCTCCGCCCCTTGTATGCCGAAAATGCTTCGAGCTTGGACACCAGACCGAAAACTGCAAACAAAAACTGACTGTCAACTCAGAAAATAATTCTGCTCAGAATGAAAAGGGTAAAAATACAAGTTTTGGTTCAGCTGAAAGTTAATTTTCTGAATGGCAGAACTTTCAACCAGAACCAAAA	CTGATATTATATCTTCTAATTTATCCATATAGAATTATTCCTTAATATATTATTTTTCGTTACGGCAGAGAAAGTCCTTCCGGAAAATCCCCGAGCTCGATCGAATTTTATATCGTCATTATGCTTTTGGTATGTCATTTATACATTCATATATTTTTTACACCTTTTCACGATTATTCAACAAATACAGGTATACCATA

 

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