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CRIMIC CR02425

Gene CG31706

CG31706

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	2L:11529038..11531052	32F2	FBti0214931	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCTATGGTACGTATGGTGTCGGTG	ACCACCAAAGATGCCCATGATG

Images

Construct Design Sequences

sgRNA sequence	CAATAGTAGTTACGTTAGCTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATGCCCAACCACCCGTCGGAATGAATCGAGGAATCGGTGAGTACCTTGATATTAAAAGCTTCGATTTAAATAGCATTGGAAAATCAGAAAAGGCAAAAGACCATTAAGCATATACATATAATCGATATAAGACTTATTAGGTTGCTAAATAAGTGACTACATTTATTTATTATGTGTGTGGCTCAATAGTAGTTACGTTA	GCTTGGACGACGTTTTCCTTAACTTCCTTACTTGTAGTGAACTAGATAGCTTGAATTAAAATCTGTAAATTTTTGTAAATTCATAGCTTTAACAATGCAGCTTTATTAATGGAAACAGATTGTTACTTTTCTAATGCAATTAATTGCTCTCACATTGTCTAGTTTCTAATCTACTTTGACGATTTCAACCCATTTTACTT

 

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