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CRIMIC CR02427

Gene emb

CG13387

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	2L:8403573..8408853	29C1-29C3		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCGAGTTCATGAAGGACACAAACTC	GCTGCAGATCACAAATGATGCTGC

Images

Construct Design Sequences

sgRNA sequence	ACATTCATTCCTGATTATCATGG
sgRNA2 sequence

 

left_200bp	right_200bp
CTTACCCATTTGGACTCTGTGGACACGGACCGCATCATGACGCTAAAGCTGCTTAATCAGGTGAACGGATCGGAATTCTCTTGGAAGAACCTGAACACGTTGTGCTGGGCCATTGGTTCCATATCCGGTGAGCCATTTTTGGTGTTTTATTCCCAGTCTGTACTTGGTTTTTATAAATCACAAACATTCATTCCTGATTA	TCATGGACCACCATTATTTCCGAGTATAACCCTATTTTTTTTTGTGCTTTTATCACTCTTCAGGTGCTTTTTGTGAAGAGGACGAAAAACGGTTCCTGGTCACCGTGATCAAGGATCTATTAGGCCTGTGCGAACAGAAGAAGGGCAAGGACAACAAGGCCATTATTGCCTCCAATATCATGTATGTGGTGGGACAGTAT

 

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