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CRIMIC CR02428

Gene MED15

CG4184

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	2L:473013..476170	21D1	FBti0214932	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCTCCAAGTGCCTCGATCAACACT	TTGCTACAGGACTTTGCAGCGTTG

Images

Construct Design Sequences

sgRNA sequence	GGTTAGTTAGTCCTTGCATTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
GTACATTGAGCCACTGAAGCGGATGCTTGCCAAGATCAGTAACGACGGAACCAGTAAGTAGATAATACCCTCACGTTTCGTTTTTTTCGCAGCTATTGCATTTTTAAATCGTTTGCTGATTACTGAAATGCTGTAAATGCTATAAACCGCGTCAAATAATATAATTTAAGAATTATATAATCGTTTTTGCGAGGCCAAAT	GCAAGGACTAACTAACCAAAAACAAAAGTTTAATGCAAACTTACTCGGGTTTATATATTTCCTAGTAGTTATATTCTCGAAGTATATGTTATCGTAAAACTAATATTATATCTTCTTTAATAGATGTGGAGAAGATGACAAAGATGAGCAAGTTGTTGGAGATTCTCTGCAATCCTACACAGCGCGTGCCGCTGGAGACT

 

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