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CRIMIC CR02433

Gene RhoGAP54D

CG6477

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	2R:17586425..17590884	54C11-54C12	FBti0214936	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGAAGTCCAAATGCACGACTTACC	GACGGTCAACACGGTGTACACTTT

Images

Construct Design Sequences

sgRNA sequence	TAAGTTTGCACATAATAACGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
CAAGCAAAATGGGCAGGGTAAATTACTTTATATATTTAATGCTTTAATGCCCTTTCACTCTGGGTCTTATGATTTAAATACCTCTGCTAGTAATTTCAAAATTACTTGAATTAAGGTTCAAAATATCTTGAATCTAATTAATATAGTTAATATTACGGAAGTAAGAAATCTATAGCCAATGTTTAAGTTTGCACATAATA	ACGAGGTAATTTTTGGGAAAAAGTTAAAATAATTAAAAAAAATATCATTATGATTTATCAACTAATCAATTTTCATTTGATTAGGCACACCGTTGCAACTGGTGGTAATGAATCGTTTGAAGAACAACGAGGCCACTCGAAGTGCCAAGTCCCTGGGAGATTCCATTAAGAAGCACATTTTCCACAAGAGCCTCATGTCG

 

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