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CRIMIC CR02445

Gene Idgf3

CG4559

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	2L:16450907..16455194	36A1	FBti0216183	92668

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GCTCTGCGTGGAAGAGTTTCAAGA	CACTCTCTGAAGCAACCAGTGTTC

Images

Construct Design Sequences

sgRNA sequence	AACAAAGTGGCTTGTATCAGTGG
sgRNA2 sequence

 

left_200bp	right_200bp
GGTGACTGCACTGATCAAGGATTTGAGTGCCGCTCTGAAACAAAATGATCTGCTGCTCAGTCTCACCGTTTTGCCAAATGTGAACTCCAGCTGTAAGTATACAAACAACAGGCAATTACTTTGTTTATTCCGACGATCTGGTGTAATTTGATGAGTCAGAAAAGTTTTTTATTTGCGTTGTGTATCGATGAAACCCACTG	ATACAAGCCACTTTGTTTAATTGGTACACAACTGTTAATTGTTTTGAAAGCAGCTGTTGAAACAGATTGTCTTAGAAAGAAGCAGGGAAAGAGTTATTATATAATGAGTATTAATGTATTACGGAACTAATAATTCAGTGCACAATTCCACTTTCTAGGGTACTATGATGCCCCGTCGATCGCCCCAAGCCTGGACTTTA

 

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