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CRIMIC CR02446

Gene Sec71

CG7578

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	2L:13793521..13799674	34D1	FBti0214940	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GCATGGAGAATCAAGTGTACGAGC	CAGATGCAATGACAGCACCTTGGA

Images

Construct Design Sequences

sgRNA sequence	TAAAATATTTCTTATCGGATGGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCGGGATTCCGACGAAGTAATTGCCTCGGAACTGCTGGCGGAGATCATATCAGGTGCGTGGCACTCGGTGGAGAAAGTTTGATAAACAGATGTTTCTTGTTAAGCTGAAGTTGCTTATTATTCTACTTAACTCGCTTAGATTTAACTATTTTTCAATATTTTCAAGAAACGTAATTAAGTTCATAAAATATTTCTTATCG	GATGGGAAAAAATTGTATGAATCATTTACAGTACGATACGATAAGGTCCAACATAAAATTCCACTCCTCCAAACCGTCTAATTATCATTGCGCGAAAATAATGACAGTTACGTTTTTCAGCTGCCTACAATGAGGCGATGAAGGATCAGGAATCGGTCGGTGAGCCAGAGCCAACACTTAATGGAAACGACTACTCCTCG

 

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