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CRIMIC CR02454

Gene CG42319

CG42319

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	2R:13260101..13266336	49F13-49F15	FBti0216187	92672

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGCTTCCCAAATCAGTTCATTCGC	TGCAATCGTGTGGTTGGTTCACCT

Images

Construct Design Sequences

sgRNA sequence	ATTGAATGATACTTAGTAATAGG
sgRNA2 sequence

 

left_200bp	right_200bp
TGTTTTACTTTCATTAATAACCCAAGTGCAATTAATAATATGTGATGTGTGTTTGATAAGTTACTAAGAAAATATCACTTAATGCTAGAAAAATATATATCTAAGTTAAAAGATGATTCAGTCAGCTTATAGTTATTTTCAATTAAGATTAAATAAACTTCCATAAATAATGAAATACAATACATTGAATGATACTTAGT	AATAGGAGAATGAAAAATAAAGAAACTCATTTACAGCCTCTCTTCTAACTGCTTTATAAGAATTCACTTGTCTTAAATGTATAACGTTCGTATCAGAATTAATTAACAATTAATGTAAATATGTAGTCATTAGGTTTCATAGTCCCCAGTTGTTTCCTGTGTAGAGCCCCGAACTCACTTGGCTTTGTATCTTTTTGTAG

 

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