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CRIMIC CR02458

Gene CG31784

CG31784

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	2L:17123307..17125961	36C1	FBti0216189	92674

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
weak band	OK	GGTTGCATCGTAGTCCAGCAACAA	TTGAAGCGAAGGTCAGGGAAACTC

Images

Construct Design Sequences

sgRNA sequence	AAATTATGTATCTTCAATTAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTGGCACTATATTCTTTTCATCATATTAGAAATCCGAAAAATTGGAAAATCTTGGAGATGGCCTCCGTGTCCTTGAATATATTTCTAATCAAGCCCGAAACAACGCTCTGAGGCTTAAAATTAAAGGTAAGTCTTTTTACATCATTTAGAATTTAATCTATTTTTGCATTGATCGCTTTTACAAAATATTTTATCCTTAA	TTGAAGATACATAATTTATGTTATAAGATACTCGGTAATTTATTTTTGTATTTATAGAAAAGGAATTAGAACTTAAGCGGTTTCGCGACCGCAAAACCTATGACGTCCATGCGTTAGCCCACTTGAAGAACAAGAAGAAAATAAACGAGGAACTGCTGAATAATATGAAATCAAAGCTAAGAACGCAGCAAAAGCTTGAA

 

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