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CRIMIC CR02462

Gene CG8353

CG8353

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	2L:8214045..8215239	28F3	FBti0214944	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTCCATTTGCCATCGGACGTTCAA	TTTCAGCAATCCTCGCATCGATCG

Images

Construct Design Sequences

sgRNA sequence	AGATCTTGTCAATGAGTGTAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
ACTATAATGACACATCTGACAAAAAATTTCGCACGTCCAGAAAAGAATGAGGAGGTTGTGACCTTCGGCAGTCTGGGTAAGATTCCTTCTCGAGATTGAGAACAATCTGAGAACAATCCTTAAATATCTACGACTGTCAATTATTCAAAGTGGTTCTTATGATATTAAAAATAATAATGTTATAAATTAATACTCCTTAC	ACTCATTGACAAGATCTAAGTTTAATTCTTGAGCGTGGCGTGCATTGTTTCGACTTCCAGATCCGTCGGTTCAAGAACTTCTGACAGCTGCTTTTCAAGTGCGCCAGCGGGCATACGTTCCATATAGTGGCTTCAAAGTTGGGGCTGCTTTTCGGGCCAAGGTCGATGGAAAGATCTTCACTGGCTGCAATGTGGAAAAC

 

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