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CRIMIC CR02479

Gene Myd88

CG2078

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	2R:9302823..9308722	45C5	FBti0216193	92678

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTTCATGATGAAGCGCTGGGTGT	TTTGCACAATGGGGATTCACCCAG

Images

Construct Design Sequences

sgRNA sequence	GAAAAGATCATTCTAGTAAGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATGCTTAAGGTATAAAAAAGCACACATAATTAAAATCGTATTAGGAATTTTTCAAAGGAAATGTTTGAATTCCTTTTCTAATGATCTTTCATTGGGTGTTTTAGAAATATTCCAATAAAACTAAAAATAACTAAAAAAGTAAATTTTTTATAAATGTGTCGTTGATGATAACTGCTTGAATCTAGAATTCACTACCTCTT	ACTAGAATGATCTTTTCTGCTGACAATATATTTGGAATAAAAAGATGGGGAGACAATGGCAGACATCTGAATGGATATATGCATACACTTATCTATTCATACATAATATTGAGTTACAAATCATAAATACTACCAATTTGTTATGAAAGCTTCATATATTAGCGACTGTGTGCAGAAACTCAGATAAACTTCACTATGCC

 

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