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CRIMIC CR02481

Gene Pgant3

CG4445

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p2	2R:7057654..7061732	42E7	FBti0214950	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTGATGGGCTGGAACAGGTTGAT	AATGCAAGTGTTCCAGTCGAGTGC

Images

Construct Design Sequences

sgRNA sequence	TATTGATGACTAAGGGTAACTGG
sgRNA2 sequence

 

left_200bp	right_200bp
CCCGCACAGTCATCCGAAACCGCCGCCCAAGCGCCGCTCCCTCTGGCCGCACAAAAACATCGTGGCCCACTACATCGGCAAGGGAGACATCTTTGGCAACATGACAGCAGGTGAGTGAGATGGTTCCACCTAATCATCATCAGTTAAGATTGGTCATTGGGGTAATAAAACAGTTTATTTGTCTTTTATCATTCCCAGTT	ACCCTTAGTCATCAATAATAGACAGTGCCATTAAATGGCATTTCATTTTACCCTCTGCACAATTGGTTTAGGTGCTCGGGAAATATTTAAGACTTCTTTAATTACCTTAGTATCATTTTACTTTCGACGCATTAGGGAAGAACTGACGAACTTTGTTTTGAAACTACAACCTTAGTTGCACCATCGTTGATGGAAAATAC

 

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