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CRIMIC CR02482

Gene CG5867

CG5867

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	2L:13236494..13239297	34A11	FBti0214951	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCTTTGGCATACGCAGACAGACCA	ACTCGCTGATGTTGATGTCACCCT

Images

Construct Design Sequences

sgRNA sequence	GGAGAAGTCTACCTTACCGGCGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTTTGGCCAAAAATAAACACACAGTCATTTAAATTACCTCATTGTCCAGGCCACAATTGCAAATGTTTTTTTATACGATATTCGCCGACCGAGTCACTTGCTGTTGTAGATATAAATATAGCTTTTTTATGGTAGAAGTTAAAAATATATGACAAGTTCGCCGCTTAAGTCTTTTTTTGCCGGACATGTCACTTCCGCCG	GTAAGGTAGACTTCTCCCAAGGTAGACCAATAGGAAAATTGCCGAGACGAACTTGAGACAACGAGCAGAACGAAACTCTTAAATGATATGCATGAATTTTCGCTCAACTCACTGACAAAATCAAATCTAATCAACAAATTAATTGCGCATGACGTTGACAATTATGTGAGCGACGAAACGTTTAAGCCAAAGTCGAACTG

 

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