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CRIMIC CR02484

Gene sha

CG13209

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	2R:11324314..11336463	47F1-47F3	FBti0214952	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGTGCAGGGCTGCAGAATGAA	GTGGTATTGTGAGCTACTTACTGGC

Images

Construct Design Sequences

sgRNA sequence	TCTTACTTTCGGTCCGACTTGGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATTATTGAACAAATCGCTTGTTTCCCCGCTGCTAATGAATGAAAGGAAACTTTCGCCCGTCGTCTGCCAACATAAACAATCTGAGACCGCTGCCATTTCGGGGTCTAACTCTAACACAGGTTCGGAATCCGACCCGAAGATCGGAATTGGAGTTGGAGTCGGAATTGGAATTGGACTTGGAATCGGAATCGGACCCCAAG	TCGGACCGAAAGTAAGAGCTGGGTTGGCCGGAAAAATAACAGCACAGATATGTAGTTTCACTACCAATTCCGATTCGTAATTGATAAGCGTCGATCGTTATCGCCGCCATTTGAACGTACTTTCCCACAACCTCCAGCACCTAATCGCTTCCCATTTTTCCGCCCATTGTTACAGAGTGCTCCCTGTCGCCATTCGTGAG

 

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