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CRIMIC CR02486

Gene CG3294

CG3294

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p2	2L:4794549..4796581	25A6	FBti0214953	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGTCACGTCTTTGTGGAGTACACA	TGTTCGGATTGCCAAACAGGTGCA

Images

Construct Design Sequences

sgRNA sequence	TATGTTAATAATGTCTAAAGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTATTATTATTTAATTAATATGACATTGTTTATATGCAAGCAATTCGGATATTCAAATGCAGCCTATTTCCCAGCATTTTCATGTAAAAGTTAATTCATATAATTCACAATTTTAATTTATACCTGTTCTCTCGCCAAAAAATATATATATTGTATTGTCTCTGCGCTTGCATACTTTTATGTTATGTTAATAATGTCTA	AAGAGGTCATAAACTAATTATTATAAAGCGTACGTCATGATTTCAGATCTGCACTTCGCGCATTTACCAATCTCCAAGGTCGCTACTACGCCTCCAAGCGACTAAACGTGGAGTTCTCCAATCTGAGGACCTGGCGCGGCGCAGTCTGCGGTACGTGAACTGCATGCCAAGAGGAAGCCGTATCCTTTAAAAAACCAAAA

 

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