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CRIMIC CR02488

Gene stc

CG3647

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p2	2L:15113480..15118069	35C2	FBti0216195	92680

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
long band	OK	AGAATCGCAGGACAAACGCACCTA	AACGACACTTTACACCGGTCTGCT

Images

Construct Design Sequences

sgRNA sequence	CAAAACATCAGAATTGAATTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
CGGTCAACCGCTTCCCTGCGGCCACCACAAGTGCAAGGACTCCTGCCACGCGGGAAGTTGTAGGTAAGAATAACTGATTCAACTCATTTATAGTATTGGTTTCATTTAAACCAATAACCCCAAAAAACGGCTCAAACTCGAAAATTGTGTTAGGTTTCTAAAATATGTGCATTAACATTAACACAAAACATCAGAATTGA	ATTTGGTATGAACCAATTTTATGCTAAATTATTCAGCGTTTGCAATCGTGTCTTTGTGCAGAGTACTAAGAAACTCATACAATTAAACTGATAATATGATTCTTTTCAGACCCTGCAAGCTGAGTCCCGAGCAGATAACAAGTTGTCCTTGTGGCAAAATGCCTGTACCGGCTGGCCAGCGTTCAAGCTGCCTGGACCCG

 

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