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CRIMIC CR02490

Gene Zir

CG11376

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p2	2L:94739..102086	21B2	FBti0214954	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCAACGAGAACTGGAACGTAACAACG	CTGCACCTCCTTGTAGCTTGAAAC

Images

Construct Design Sequences

sgRNA sequence	AAGTAATATCTATATTAGGCTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTGACTCCCTTTATTACTCTTACATCAGTTCAGAATCATGGCGTTGTCAAGTAAGTTTTTATTGTTATACAGTGAAAAGGAAAGTTACATTGTGTATACATATGTATTAAAATTCTCATGTCTTTCTTATTTTGTTCCTCAACGGAGTGCTCTAAATAATAGACATAAGATGCTTAATTAGATATTTATAGAATCCAGCC	TAATATAGATATTACTTTACTATATGACTATATTAAAAACCACCAGTGCAAAAAGTAAACATTAACTTATATAATTTTTTATTTCTTTTCTAAATACATCCAATAGGGCCAACGCGTACAACAACAAAAGTACTCCTTTGAAATGGTAACTTCTTGCACACGACTTAGTCTGCAATCAAATTATCATACTTTTGGTCTTT

 

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