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CRIMIC CR02492

Gene DUBAI

CG8830

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p2	2R:12431447..12436577	49A6	FBti0214956	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACCAACACTGTGACCAGTGACTGA	CTCTGGCAAAGGCAATGCATGAGA

Images

Construct Design Sequences

sgRNA sequence	ATTACGATTACGAAGCTTATTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TAAAGATCAAATGAAGAAACCCCACTTCTAATTTCCCCAAAGTAGAAGCGCAAAATAATATCTTTGCCTCATATTTATGATTAAAACTCGGTTCTATATTTGCATTGCCTTGCCAGCACCTTTTGTTTAAGAGCTCACTGTTTATAAATCTGTTTTAGGTGTCAATTTCCGATTACGAAAATAATTACGATTACGAAGCT	TATTGGCTTTATTTATCTTCTTACAACTACATATACTCATTTTCCATTTGCAGTTTTAGCAGGCAAATCCTGCTTATCGAGTGCAATTCAGTGCTGCTCATGAAAGTGCAGCAGCAGATTGCCCTGATGCATCACTCGCTGCGCTACGAACTAACGCCTTCGCGAGTGCTGAACGCCACCCGGCCGCCTAGCTTCACGCC

 

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