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CRIMIC CR02493

Gene Pgant1

CG8182

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p2	2R:15511710..15515597	52A6	FBti0214957	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GACTGTTTGCCATAGATCGTCGCT	GTTCTGCAGCAAATCGTCCAGACA

Images

Construct Design Sequences

sgRNA sequence	GTTACTATAGTTCGCTTCGAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
CTGGGAAGTGGGCAGCTACGACGAACAAATGGACGGTTGGGGTGGTGAGAACCTGGAGATGTCCTTTCGCATCTGGCAGTGCGGCGGCACCATCGAGACTATTCCCTGCTCCCGCGTTGGACACATATTCCGCGACTTCCATCCTTACAAGTAAGCGAAACGAACTATATACTTATAATTTATGATGAAGCTGACCTTCG	AAGCGAACTATAGTAACTTTATTGTCATCTGCCATTATCTATTGCTGCATTATAATGAACTCATTTAAATTCTTTATAGATTCCCCAACGATCGCGATACACACGGTATTAATACTGCCCGCATGGCTCTGGTTTGGATGGATGAGTACATCAACATATTTTTCCTCAACCGGCCGGACCTAAAGTTCCACGCGGACATT

 

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