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CRIMIC CR02494

Gene Or33b

CG16961

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p2	2L:11936181..11937501	33B2		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCCAATGATTCCTATCCACCGATG	TGGTTCATCTCCACGGATATCAGG

Images

Construct Design Sequences

sgRNA sequence	GTGACACCCGACAAACAGAATGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTGCGTGGTTGCCGGTCATGTAAGACTTTTGGCGATGCGCTTGAGTAGAATTGGCCAAGGTCCAGAGGAAACAATATACTTAACCGGAAAGCAATTAATCGAAAGCATCGAGGATCACCGAAAACTAATGAAGTAATGTACATATATAGAATGGTTTTTAGTTATTATCATTAAATGAACGTGTTGTAGGAAAACCATTC	TGTTTGTCGGGTGTCACGGAAATCGATTTTCCTTAATTTACATATGATATTAAATACTTCCTTGCAAACAATTATCATATTAGTAATTTAGAATCTTTATTATTTATTTCCAGAATAGTGGAATTACTGCGCAGCACCATGAATATTTCGCAGCTCGGCCAGTTTATTTCAAGTGGTGTTAATATTTCCATAACACTAGT

 

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