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CRIMIC CR02501

Gene Gr33a

CG17213

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p2	2L:12138008..12140392	33D2	FBti0216946	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TATCTGACCATCACCCTGCTGTCA	GTCCATGACCGTAATCGGTGTAAC

Images

Construct Design Sequences

sgRNA sequence	AAGTAATAATTATTGTAGTTCGG
sgRNA2 sequence

 

left_200bp	right_200bp
GTTATCCAGAAATGCCAGCGGGTGGCGGAAATGGACAAGGTGAAGGTTACTGCGGTGAAACACAGTGTGGCCTATCACTTCACCTGGCTTTTCCTATTCTGCGTTTTCACCTTTGCCCTTTACTATGACGTCAGATCTTTGTACTTGTGAGTATGCCGTTCAAATATACTAAAGATTAAAGAACTAAATTGTTGCCGAAC	TACAATAATTATTACTTAAATTGCAATATTTTTGCCATATCCTAATCATTGGTAGTTGAATTTTAAAGCCCAAATTGTCTCGTTCTAGGACCTTCGGCAATCTCGCCTTCATTCCGTTCATGGTGTCCAGTTTCCCATATTTGGCCGGCAGCATCATTCAGGGTGAGTTCATCTATCACGTGTCGGTCATCTCGCAGCGC

 

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