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CRIMIC CR02514

Gene NimB1

CG33119

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p2	2L:13963507..13965088	34E5	FBti0214965	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CATCGAAGCTCAGAACACCAAGAC	CAAAGTTCTGATGGCAGACACAGG

Images

Construct Design Sequences

sgRNA sequence	TATTTATTTGAGCGCATGCTGGG
sgRNA2 sequence

 

left_200bp	right_200bp
TCTACGGCGACATAGAGAATACGGAGTACGGCGATTTTGAGTCACTAAGTCAAACTCGCGAGCGTCAGCAACATCTGTGCCATCGCGAGGTGCCGTGAGTAAAAGGAAGTTTTAAAAAATATGGAAAATAAAACAAAGCAGCTTAATGCCAAACAAACGTTCATTAATAAACAAATATATATATATTTATTTGAGCGCAT	GCTGGGAGAAATGATTACAAGCGCCCATTGATTAATTCGATTTGATGATTACAGTTCTGTGTTTTTCCAAACCGAGCGGGATTCCCCGGTGCGTGGTAATGGATCCACGATCTACTTCCATCGCATAGAGGTCTGTTGTGCAGGCTACCGTCGCGATCCGTATGCCAATGAATGTGTGCCGGACTGCTCCGCATCTTCGC

 

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